Mold Assessment Technician Practice Exam

Which method is used for evaluating non-viable air samples?

1. Incubation before analysis

2. Direct examination under a microscope

3. Sampling with a water medium

4. Absorption on growth media

The correct answer is: Direct examination under a microscope

Evaluating non-viable air samples typically involves direct examination under a microscope. This method allows for the identification of mold spores and other airborne particulates without the need for the growth of the organism, which is not applicable for non-viable samples. In this context, non-viable refers to mold spores that are not capable of reproduction or growth, necessitating techniques that provide immediate analysis without fostering growth. In contrast, incubation before analysis is more suitable for viable samples, as this technique relies on allowing viable spores to grow and form colonies before assessment. Sampling with a water medium is not effective for air samples since it is more aligned with capturing particulates in aquatic environments, which do not mimic typical air sampling conditions. Absorption on growth media is also inappropriate for non-viable samples since it requires the organism to be alive and capable of growth to form colonies that can then be counted or analyzed. Thus, the method of direct examination under a microscope is the most accurate and appropriate approach for evaluating non-viable air samples, providing immediate insights into the presence and identity of the spores present in the air.